Coding

Part:BBa_K895005:Design

Designed by: Frank Sargent   Group: iGEM12_Dundee   (2012-09-23)

Salty_VgrG-CD27_endolysin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 474
    Illegal BglII site found at 1806
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1048
    Illegal AgeI site found at 1195
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.


Source

The vgrG section is derived from BBa_K895002, which was used as a PCR template, and the endolysin section is derived from a synthetic gene designed by Neil Fairweather's group at Imperial College London.

References

The Salmonella Type VI Secretion System is reviewed by these guys:

Blondel,C.J., Jimenez,C.J., Contreras,I. and Santiviago,C.A. (2009) Comparative genomic analysis uncovers three novel loci encoding type six secretion systems differentially distributed in Salmonella serotypes. BMC Genomics 10:354.

The bacteriophage CD27 endolysin against C. difficile is described here:

Mayer,M.J., Narbad,A. and Gasson,M.J. (2008) Molecular characterization of a Clostridium difficile bacteriophage and its cloned biologically active endolysin. J. Bacteriol. 190:6734-6740.